---
title: "Rutin Alleviates Oxidative Stress in Chicken Hepatocytes by Inhibiting Ribotoxic Stress Response"
authors: ["Cangning Zhang", "Tao Li", "Chengfeng Zhao", "Liqin Zhao", "Duniesky Rodriguez Acosta", "Osmany Chacon Chacon", "Liumei Sun", "Weiguo Zhao", "Liang Qu", "Manman Shen"]
journal: "Animals"
published_date: "2026-05-28"
doi: "10.3390/ani16111654"
url: "https://doi.org/10.3390/ani16111654"
source: "crossref:crossref-animals"
fetched_at: "2026-05-30T18:53:30+00:00"
tags: ["行为识别"]
relevance_score: 1.0
reading_status: "unread"
favorite: false
---

# Rutin Alleviates Oxidative Stress in Chicken Hepatocytes by Inhibiting Ribotoxic Stress Response

## 基本信息
- 作者：Cangning Zhang; Tao Li; Chengfeng Zhao; Liqin Zhao; Duniesky Rodriguez Acosta; Osmany Chacon Chacon; Liumei Sun; Weiguo Zhao; Liang Qu; Manman Shen
- 期刊：Animals
- 发表日期：2026-05-28
- DOI：10.3390/ani16111654
- 原文链接：https://doi.org/10.3390/ani16111654
- 数据来源：crossref:crossref-animals

## 摘要
Oxidative stress (OS) significantly impairs poultry production; however, the protective mechanisms of rutin in the chicken liver remain unclear. In this study, we investigated the regulatory effects of rutin on oxidative stress and lipid metabolism using H2O2-exposed primary hepatocytes derived from chicken embryos. Specifically, we focused on antioxidant indicators, the Nrf2/Keap1 signaling pathway, and the ZAKα-mediated ribotoxic stress response (RSR). Results showed that pretreatment with 800 μmol/L rutin significantly alleviated oxidative damage by increasing superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) activities, while decreasing malondialdehyde (MDA) and reactive oxygen species (ROS) levels. This protective effect was mediated by activation of the Nrf2/Keap1 signaling pathway and its downstream effectors, HO-1 and SOD2. Concurrently, rutin suppressed lipid accumulation, as evidenced by reduced levels of triglyceride (TG), total cholesterol (T-CHO), and low-density lipoprotein cholesterol (LDL-C), along with the downregulation of SREBP1 expression. Mechanistically, we found that rutin inhibited ZAKα and its downstream signaling, the p38/JNK pathway, which is associated with RSR. Crucially, co-treatment with ZAKα overexpression significantly attenuated the protective effects of rutin on both antioxidant defense and lipid regulation, confirming that rutin functions by inhibiting ZAKα-mediated signaling to restore Nrf2 activity. Molecular docking revealed a binding energy of −8.6 kcal/mol between rutin and ZAKα, verifying that ZAKα serves as a potential target for rutin. Collectively, these findings elucidate a novel mechanism whereby rutin enhances antioxidant defense and inhibits lipid deposition via the ZAKα-p38/JNK-Nrf2 signaling pathway, providing a theoretical basis for the application of rutin as a targeted regulator in poultry health.

## 中文整理
基础摘要（未启用或未成功调用大模型）：Oxidative stress (OS) significantly impairs poultry production; however, the protective mechanisms of rutin in the chicken liver remain unclear. In this study, we investigated the regulatory effects of rutin on oxidative stress and lipid metabolism using H2O2-exposed primary hepatocytes derived from chicken embryos. Specifically, we focused on antioxidant indicators, the Nrf2/Keap1 signaling pathway, and the ZAKα-mediated ribotoxic stress response (RSR). Results showed that pretreatment with 800 μmol/L rutin significantly alleviated oxidative damage by increasing superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) activities, while decreasing malondialdehyde (MDA) and reactive

## 关键词标签
行为识别

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1.0

## 相关性说明
命中 行为识别 关键词：activity

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