Carboxymethyl β-cyclodextrin-loaded buffalo milk peptide nanoparticles ameliorate DSS-induced intestinal mucosal injury in mice.

基本信息

• 作者：Qiong Zhao; Chenghao Zhang; Yinan Feng; Boxi Xu; Wentao Zheng; Guangqiang Wei; Aixiang Huang
• DOI：10.3168/jds.2025-28156
• 原文链接：https://doi.org/10.3168/jds.2025-28156
• 数据来源：pubmed:pubmed-jds
• 抓取时间：2026-05-30T18:53:27+00:00
• Markdown 文件：/root/worksplace/paper-tracker/exports/obsidian/2026-05-09-carboxymethyl-β-cyclodextrin-loaded-buffalo-milk-peptide-nanoparticles-ameliorate-dss-induced-intestinal-muco.md

摘要

Buffalo milk-derived active peptides have a variety of important regulatory functions for the body, but their poor oral stability leads to low bioavailability. Herein, in vivo stability of peptides DN8 and YR9 screened from buffalo milk were enhanced using encapsulation, and the effects of these peptides on ameliorating intestinal mucosal injury in mice were investigated. The binding of peptides DN8 and YR9 to CMβ-CD was mainly through electrostatic adsorption and hydrogen bonding forces to form robust nanoparticles, associated with changes in O-H, C = O, and C-H bonds. The average particle size of NPs CMβ-CD-DN8 and CMβ-CD-YR9 were 75.03 nm and 41.71 nm, and the encapsulation rates were 82.513 ± 4.617 and 90.974 ± 0.421, respectively. NP CMβ-CD-DN8 and CMβ-CD-YR9 demonstrated stability and non-hemolytic properties at the tested concentrations in vitro, and effectively reduced the secretion of pro-inflammatory cytokines NO, IL-6, and TNF-α in LPS-stimulated RAW264.7 cells. Peptides DN8 and YR9 in NPs were released in small amounts in the stomach but rapidly in the intestine. Furthermore, the NPs CMβ-CD-DN8 and CMβ-CD-YR9 were found to be effective in attenuating DSS-induced epithelial barrier injury in mice by increasing the expression of Occludin and ZO-1. This research provides insights for the development of oral food-borne active peptides with high bioavailability.

中文整理

基础摘要（未启用或未成功调用大模型）：Buffalo milk-derived active peptides have a variety of important regulatory functions for the body, but their poor oral stability leads to low bioavailability. Herein, in vivo stability of peptides DN8 and YR9 screened from buffalo milk were enhanced using encapsulation, and the effects of these peptides on ameliorating intestinal mucosal injury in mice were investigated. The binding of peptides DN8 and YR9 to CMβ-CD was mainly through electrostatic adsorption and hydrogen bonding forces to form robust nanoparticles, associated with changes in O-H, C = O, and C-H bonds. The average particle size of NPs CMβ-CD-DN8 and CMβ-CD-YR9 were 75.03 nm and 41.71 nm, and the encapsulation rates were 82.

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未命中核心研究方向关键词，建议人工复核。