Dose-response of a fibrolytic enzyme in dairy cows fed a sorghum silage-based diet: effects on productive performance, ruminal fermentation, and in vitro gas kinetics.

基本信息

• 作者：Catalina Rivoir; Anaclara Daudet; María A Bruni; Pablo Chilibroste; Valentin Nenov; Lode Nollet; Alberto Casal
• DOI：10.3168/jds.2025-28044
• 原文链接：https://doi.org/10.3168/jds.2025-28044
• 数据来源：pubmed:pubmed-jds
• 抓取时间：2026-05-30T18:53:27+00:00
• Markdown 文件：/root/worksplace/paper-tracker/exports/obsidian/2026-05-18-dose-response-of-a-fibrolytic-enzyme-in-dairy-cows-fed-a-sorghum-silage-based-diet-effects-on-productive-perf.md

摘要

The objective of this study was to evaluate the effect of different doses of an exogenous fibrolytic enzyme (EFE) product, containing xylanase with secondary cellulase activity, derived from Trichoderma citrinoviride, on productive performance and ruminal fermentation in vivo, and on gas production kinetics and nutrient digestibility in vitro. Sixteen multiparous, mid-lactation dairy cows fed a TMR based on sorghum silage were used in the in vivo experiment following a 4 × 4 Latin square design. Cows were randomly assigned to 4 treatment sequences based on the total daily EFE dose top-dressed onto the TMR (g of product/cow/d): 0 (T0, control), 5 (T5), 10 (T10), and 15 (T15). Milk yield showed a quadratic response, peaking at T10. Ammonia (N-NH 3 ) concentration in rumen fluid decreased following a quadratic pattern with increasing dose, and VFA concentration showed a cubic response. Gas production (mL gas/g of DM and OM incubated) increased linearly with dose, as well as the maximum gas production parameter. Gas production rate and lag time were not affected by treatment. Organic matter, DM and NDF digestibility at 48 and 96 h increased following a linear and cubic pattern with EFE doses. Further research is warranted to elucidate the mechanisms of action of EFE in dairy cows fed sorghum silage-based diets.

中文整理

基础摘要（未启用或未成功调用大模型）：The objective of this study was to evaluate the effect of different doses of an exogenous fibrolytic enzyme (EFE) product, containing xylanase with secondary cellulase activity, derived from Trichoderma citrinoviride, on productive performance and ruminal fermentation in vivo, and on gas production kinetics and nutrient digestibility in vitro. Sixteen multiparous, mid-lactation dairy cows fed a TMR based on sorghum silage were used in the in vivo experiment following a 4 × 4 Latin square design. Cows were randomly assigned to 4 treatment sequences based on the total daily EFE dose top-dressed onto the TMR (g of product/cow/d): 0 (T0, control), 5 (T5), 10 (T10), and 15 (T15). Milk yield showe

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相关性说明

命中 奶牛/牛只 关键词：dairy cow, cow；命中 行为识别 关键词：activity